Chapter #20: Electrospinning and Three-Dimensional (3D) Printing for Biofabrication (pdf)
Nureddin Ashammakhi, Maryam Tavafoghi, Arman Jafari, Sumama Nuthana Kalva, Robin Augustine, Anwarul Hasan, Houman Savoji, Yavuz Nuri Ertas, Song Li
Biofabrication of engineered cell-laden constructs and scaffolds is essential for tissue engineering and tissue modeling. Electrospinning is a highly scalable technology to fabricate porous scaffolds with micro or nano-fibrous structure. Three-dimensional (3D) bioprinting has been recently developed for tissue engineering by providing control over cell location and multicellular structure. With the availability of electrospinning techniques, it is possible to combine nano- and microfiber-based structures with 3D bioprinted constructs, to obtain composite structures that have biomimetic or functional features and improved mechanical stability. In addition, electrospun fibers can add various functions such as drug release properties to the developed 3D bioprinted constructs. In this chapter, we will discuss the techniques for electrospinning, 3D bioprinting, and the approach of combining electrospinning and 3D bioprinting for biofabrication. We also highlight current challenges and future research directions.
Chapter #13: In Situ Tissue Engineering: A New Dimension (pdf)
Yavuz Nuri Ertas*, Asma Sadat Vaziri, Keyvan Abedi-Dorcheh, Fereshteh Kazemi-Aghdam, Masoume Sohrabinejad, Rumeysa Tutar, Fatemeh Rastegar-Adib, Nureddin Ashammakhi
Tissue engineering has evolved to provide ways to construct tissues primarily aiming at replacing lost or damaged tissues or improving function. It has been classically developed using ex vivo means in which cells are generally cultured with biomaterials and subsequently engineered constructs are transplanted into the body. However, this approach is associated with several challenges that have limited its successful translation to the clinic. With in situ tissue engineering, it is possible to stimulate internal body regenerative potential by using biomaterials, biomolecules, and genes, which can reduce risks and challenges associated with ex vivo tissue engineering. In addition, in situ tissue engineering may potentially accelerate the clinical application of the technology and may lead to the development of more effective regenerative therapeutics through a collaborative multidisciplinary approach.